As a result of successfully completing this chapter, readers will be able to:
Define the term, “disinfection,” and explain how disinfection differs from sterilization.
Review factors that impact the effectiveness of a disinfectant.
Discuss the relationship between the risk level (intended use) of the device to be disinfected and the selection of a disinfectant.
Explain disinfectant activity levels as they relate to the resistance of microorganisms to germicidal agents:
• high-level disinfection
• intermediate-level disinfection
• low-level disinfection
Review factors that affect the chemical action of and other important selection considerations for disinfection.
• Provide basic information about the types of disinfectants commonly used in healthcare facilities: quaternary ammonium compounds, phenolics, alcohol, halogens, glutaraldehyde, ortho-phthalaldehyde, and formaldehyde.
• Review safety requirements that should be followed when using chemical disinfectants.


